Morphic larvae. Functional annotation, according to both Gene Ontology and KEGG Pathway analyses, of genes significantly up-regulated in pre-metamorphic larvae and positively correlated to PCA Y-axis projection. Further file 7: List and annotation of up-regulated genes on pre-metamorphic larvae. List and annotation of 372 genes identified both substantially up-regulated in pre-metamorphic larvae and positively correlated to samples projection on PCA Y-axis. Added file eight: List and annotation of down-regulated genes on pre-metamorphic larvae. List and annotation of 190 genes located both considerably down-regulated in pre-metamorphic larvae and negatively correlated to samples projection PCA Y-axis. Competing interests The authors declare that they’ve no competing interests. Authors’ contributions PPG, LB, SC, PM and AB conceived and created the project. AB and LP carried out fish rearing and sampling. SF carried out transcriptome sequencing and annotation, probe design and style, microarray experiments and qRTPCR validation. SF and LB executed statistical analyses and functional annotation. SF, LB, AB and PPG wrote the manuscript. All listed authors edited the manuscript. All authors read and authorized the manuscript. Acknowledgements Study project was partially funded by the Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia. We thank also two anonymous reviewers for their useful comments on an earlier version from the manuscript. Author details 1 Division of Comparative Biomedicine and Food Science, University of Padova, Viale dell’Universit?16, Legnaro, PD 35020, Italy. 2Department of Veterinary Health-related Sciences, Alma Mater Studiorum, University of Bologna, by means of Tolara di Sopra 50, Ozzano Emilia, BO 40064, Italy. 3Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia, through Bianchi 9Brescia 24125, Italy. four Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia, Sezione di Forl? By way of Marchini 1, Forl?47122, Italy. Received: 18 October 2012 Accepted: 1 May 2013 Published: 10 Could 2013 References 1. Okada N, Takagi Y, Seikai T, Tanaka M, Tagawa M: Asymmetrical improvement of bones and soft tissues for the duration of eye migration of metamorphosing Japanese flounder, Paralichthys olivaceus.6-Bromo-2-methylpyrimidin-4-amine Chemscene Cell Tissue Res 2001, 304(1):59?6. 2. Cerd?J, Douglas S, Reith M: Genomic resources for flatfish investigation and their applications. J Fish Biol 2010, 77:1045?070.three. four.5.six.7.eight.9.10.11.12.13. 14. 15.16.17.18.19. 20.21.Mazurais D, Darias M, Zambonino-Infante JL, Cahu CL: Transcriptomics for understanding marine fish larval improvement. Can J Zool 2011, 89:599?11. Bonaldo A, Parma L, Badiani A, Serratore P, Gatta PP: Very early weaning of widespread sole (Solea solea L.) larvae by indicates of diverse feeding regimes and 3 industrial microdiets: influence on performances, metamorphosis improvement and tank hygiene.886779-77-7 uses Acquaculture 2011, 321:237?44.PMID:34856019 Agulleiro MJ, Nguis V, Ca vate JP, Mart ez-Rodr uez G, Mylonas CC, Cerd?J: Induction of spawning of captive-reared Senegal sole (Solea senegalensis) using distinct delivery systems for gonadotropin-releasing hormone agonist. Aquaculture 2006, 257(1?):511?24. Concei o LEC, Ribeiro L, Engrola S, Arag C, Morais S, Lacuisse M, Soares F, Dinis MT: Nutritional physiology for the duration of improvement of Senegalese sole (Solea senegalensis). Aquaculture 2007, 268(1?):64?1. Cerd?J, Douglas SE, Buesa C, Ca vate P, L ez-Barea J, Manchado M, Mart ez G, Navas JI, Piferrer F, Planas J, Prat F, Reith M, Ru -Rej M, Y era M:.